Journal: American journal of physiology. Lung cellular and molecular physiology

Article Title: Mechanical Stretch Promotes Sustained Proliferation and Inflammation in Developing Human Airway Smooth Muscle

doi: 10.1152/ajplung.00070.2025

Figure Lengend Snippet: The effect of stretch in Piezo protein expression in fASM cells. ( A , B) : fASM cells grown on Flexcell plates were exposed to 5%, 10% or 15% of static stretch for 24h. Piezo1 and Piezo2 protein expression was determined by JESS assays. The expression levels in stretched cells were normalized to that of control cells without stretch treatment. Representative JESS images are shown in ( C ). SS: static stretch. Total protein signal values (x 10 5 ) are shown at the bottom of each lane. ( D , E) : fASM cells grown on Flexcell plates were exposed to 5% or 10% of static stretch superimposed on 5% oscillatory stretch (CPAP) for 24h. The Piezo1 and Piezo2 protein expression levels in these cells were normalized to that of control cells with 5% oscillatory stretch only. Representative JESS images are shown in ( F ). Data are expressed as means ± SD (n = 6–7 cell lines, each dot represents one cell line). Open symbol: male. Solid symbol: female. All data pass Shapiro-Wilk Normality tests. For statistical analysis, paired t tests are applied to control and treatment groups. Exact P value is above the data in Fig. 1A .

Article Snippet: In some experiments, fASM cells were treated with Piezo1 antagonist GsMTx4 (1 μM, MedChemExpress, HY-P1410) or Piezo2 antagonist D-GsMTx4 (1 μM, MedChemExpress, HY-P1410B) for one hour prior to stretch and the treatments continued thereafter during the stretch protocol.

Techniques: Expressing, Control

Journal: American journal of physiology. Lung cellular and molecular physiology

Article Title: Mechanical Stretch Promotes Sustained Proliferation and Inflammation in Developing Human Airway Smooth Muscle

doi: 10.1152/ajplung.00070.2025

Figure Lengend Snippet: The long-term effect of stretch in fASM cell proliferation. fASM cells on Flexcell plates were pretreated with inhibitors to Piezo1 ( A ) or Piezo2 ( B ) for 1h and then were exposed to 5% static stretch with 5% oscillatory stretch (5% CPAP) with or without the inhibitors. Control cells were exposed to 5% oscillatory stretch only. Cells were harvested after 24h treatment and seeded into 96-well plates. After 3-day culture without stretch, cell proliferation was measured by CyQUANT assay. Open symbol: male. Solid symbol: female. All data pass Shapiro-Wilk Normality tests. For statistical analysis, Two-Way ANOVA with uncorrected Fisher’s LSD test are applied. Data are expressed as means ± SD (n = 6–7 cell lines, each dot represents one cell line). The interaction p-values are below the corresponding panels.

Article Snippet: In some experiments, fASM cells were treated with Piezo1 antagonist GsMTx4 (1 μM, MedChemExpress, HY-P1410) or Piezo2 antagonist D-GsMTx4 (1 μM, MedChemExpress, HY-P1410B) for one hour prior to stretch and the treatments continued thereafter during the stretch protocol.

Techniques: Control, CyQUANT Assay